蛋白酶K(凍干粉)
- 別名:Proteinase K;Endopeptidase K; Prok; Protease K;39450-01-6
- 編碼:EBY0027I
- 級別:≥30 Units/mg protein
- 簡介:蛋白酶K是一種枯草蛋白酶類的高活性蛋白酶,用于生物樣品中蛋白質的一般降解。蛋白酶K是一種切割活性較廣的絲氨酸蛋白酶。它切割脂族氨基酸和芳香族氨基酸的羧基端肽鍵。此酶經純化去除了RNA酶和DNA酶活性。由于蛋白酶K在尿素和SDS中穩定,還具有降解天然蛋白質的能力。
基礎信息
中文名 | 蛋白酶K(凍干粉) |
英文名 | Proteinase K, lyophilized powder |
CAS | 39450-01-6 |
E.C | 254-457-8 |
Mol. Weight | 29KDa |
Source | Tritirachium album limber(林伯氏白色念球菌) |
產品介紹
蛋白酶K是一種枯草蛋白酶類的高活性蛋白酶,用于生物樣品中蛋白質的一般降解。蛋白酶K是一種切割活性較廣的絲氨酸蛋白酶。它切割脂族氨基酸和芳香族氨基酸的羧基端肽鍵。此酶經純化去除了RNA酶和DNA酶活性。由于蛋白酶K在尿素和SDS中穩定,還具有降解天然蛋白質的能力。
蛋白酶K用途
- 基因診斷試劑盒
- 基因組DNA提取試劑盒
- RNA提取試劑盒中去除DNA和RNA制備中的核酸酶
- 提取組織中非蛋白成份降解含有蛋白質的雜質-譬如DNA疫苗和肝素的制備
- 制備脈沖電泳的染色體DNA
- 蛋白質印跡
- 蛋白酶K的一般工作濃度50-100ug/ml
蛋白酶K參考文獻資料
1. 【產品推薦】蛋白酶K (源自林伯氏白色念球菌) Proteinase K,from Tritirachium album limber
2. Kraus, E; et.al. Proteinase K from the Mold Tritirachium album limber, Specificity and Mode of Action. Z. Physiol. Chem., 357:939; 1976.
3. Jany,KD, et al. Amino Acid Sequence of Proteinase K from the Mold, Tritirachium album limber. Proteinase K; a Subtilisin-related Enzyme with Disulfide Bonds. FEBS Letter, 199,139.1986.
4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Volume 3, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, B.16.
5. Sweeney, P.J. and Walker, J.M. (1993) Enzymes of molecular biology. In: Methods in Molecular Biology, Vol. 16, M.M. Burrell, ed., Humana Press, Inc., Totowa, NJ, 305.
2. Kraus, E; et.al. Proteinase K from the Mold Tritirachium album limber, Specificity and Mode of Action. Z. Physiol. Chem., 357:939; 1976.
3. Jany,KD, et al. Amino Acid Sequence of Proteinase K from the Mold, Tritirachium album limber. Proteinase K; a Subtilisin-related Enzyme with Disulfide Bonds. FEBS Letter, 199,139.1986.
4. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Volume 3, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, B.16.
5. Sweeney, P.J. and Walker, J.M. (1993) Enzymes of molecular biology. In: Methods in Molecular Biology, Vol. 16, M.M. Burrell, ed., Humana Press, Inc., Totowa, NJ, 305.
蛋白酶K相關產品
質量指標
Activity(37℃) | ≥30.0U/mg |
Solubility | Clear |
DNase | Not detected |
RNase | Not detected |
Appearance | White lyophilized powder |
MSDS
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